Study on the Hemagglutination Reaction with Special Reference to the Influence of Heating on the Antigens

s of "Tuberculosis Research" Vol. 9, 1958. Study on the Hemagglutination Reaction with Special Reference to the Influence of Heating on the Antigens Katsuo ONO and Yoshio TAKAHASHI Proteinic fractions were isolated from Sauton culture filtrates of human tubercle bacilli (H 37 Rv), by precipitation with trichloroacetic acid. Polysaccharine fractions were isolated from the deproteinized filtrates by precipitation with methanol at concentrations of 30 to 40 and 80 to 90 percent. The hemagglutination reaction was comparatively carried out with these fractions, according to MiddlebrookDubos' method and Boydon's. At the same time, influence of heating on the fractions was studied. The results were as follows: 1. According to M.-D.'s method, both polysaccharine and proteinic fractions gave positive reactions, while, according to B.'s method, only proteinic fractions did so. 2. Of the polysaccharine fractions, 80 to 90 percent methanol fractions gave positive reactions, while , below 40 percent methanol fractions did not. In the cross-inhibition test, these latter fractions showed no inhibiting power to the reactions to be given by the former fractions. 3. Dialysis (3 days, running water) and mild hydrolysis with MIlO HCl excerted no appreciable influence on the hemosensitizing power of the proteinic fractions. 4. Heating at 120°C during 2 hours decreased remarkably, especially In an alkaline medium, the hemosensitizing power of the fractions, while their precipitin and inhibiting titres remained unchanged. Study on the Blood Level of Isoniazid in Case of Combined Chemotherapy Tadazumi ONODERA, Tadashi Kuw AJIMA, Hajime FUKAE and Eiichi SAKAI In order to get information of the mechanism of the effect of the current, various procedures of combined chemotherapy for tuberculosis, the blood level of INH was measured with sera obtained respectively from patients treated with INH alone, those treated with INH plus PAS, those treated with INH plus pyrazinamide (PZA) andthose treated with INH plus sulfisoxazole. The measurement was done according to Ogawa's diffusion method (biological assay). According to the method, no evidence was obtained that would indicate any elevation of the blood level of INH in any of the combined treatments above mentioned. In the results obtained, the individual differences were so great that it was impossible to draw any significant conclusion. As for the development of INH-resistance, bacilli of high resistance were found in patients in whom the blood level of INH was relatively high. Moreover, no correlation was found between development of INH-resistance and doses of INH administered. Study on the Mechanism of the Effect of Combined Chemotherapy of Sulfonamides and Isoniazid in Tuberculosis Patients